Dewasa ini telah ada bioetanol generasi kedua yang berbahan baku lignoselulosa. Bahan baku bioetanol ini adalah limbah kertas HVS yang diproses melalui hidrolisis dan fermentasi. Tujuan hidrolisis adalah mengkonversi selulosa menjadi gula. Hidrolisis dilakukan pada suhu 90℃ selama 150 menit. Variasi katalis H2SO4 yang digunakan ialah 2, 3, 4, 5, 6, dan 7% dengan ratio kertas HVS : air 1:8. Konsentrasi gula maksimum sebesar 10,63% dihasilkan dari penggunaan H2SO4 7%. Variasi ratio kertas HVS : air yang digunakan ialah 1:7 dan 1:8 dengan H2SO4 7%. Ratio 1:8 merupakan ratio optimum yang menghasilkan konsentrasi gula maksimum sebesar 10,63%. Hidrolisat ditambahkan NaOH 50% (b/v) agar mencapai pH fermentasi. Penambahan NaOH mengakibatkan kenaikan kadar gula dari kisaran 9,68-10,63% menjadi 11,82-13,25%. Fermentasi bertujuan untuk mengkonversi gula menjadi bioetanol. Fermentasi dilakukan pada suhu 35℃, pH 4,5 dengan 100 rpm selama 48 jam. Hidrolisat yang telah ditambahkan glukosa kadar gulanya meningkat hingga 14,91%, setelah dilakukan fermentasi menggunakan fermipan 10% (v/v) menghasilkan etanol 1,58%. Setelah dilakukan distilasi pada suhu ±80℃ selama 1 jam kadar etanolnya meningkat menjadi 7,01% dan setelah penambahan CaO 10% (b/v) kadar etanolnya menjadi 7,78%. Fermentasi dengan variasi inokulum S.cerevisiae murni sebesar 6, 7, 8, 9, 10 dan 11% (v/v) dengan konsentrasi gula 11,82% menghasilkan etanol maksimum 0,128% pada volume inokulum 11% (v/v). Setelah didistilasi pada suhu 80℃ kadar etanolnya meningkat menjadi 0,457%. Fementasi dengan pembandingan jenis inokulum diperoleh bahwa S.cerevisiae murni 11% (v/v) menghasilkan etanol 0,12% dan fermipan 11% (v/v) menghasilkan etanol 0,07% dengan gula awal 11,82%. S.cerevisiae murni 11% (v/v) dan fermipan 11% (v/v) yang telah ditambahkan glukosa hingga kadar gulanya 17,29% menghasilkan etanol berturut-turut 0,88% dan 0,87% Kata kunci: Hidrolisis, Fermentasi, Limbah Kertas HVS, H2SO4, S.cerevisiae, Distilasi, CaO.

Nowadays was existed 2nd generation of bioethanol which used lignocellulose as a raw material. Raw material of this bioethanol was waste HVS paper which proceed by hydrolysis and fermentation. The purpose of hydrolysis to convert cellulose to sugar. Hydrolysis was carried out at 90℃ for 150 minutes. Variations of H2SO4 catalyst were 2, 3, 4, 5, 6 and 7% with the ratio of HVS paper and water 1:8. The maximum concentration of sugar was 10.63%, it was proceed by H2SO4 7%. Variations of HVS paper and water's ratio were 1:7 and 1:8 with H2SO4 7%. Ratio 1:8 was an optimum ratio which produced the maximum concentration of sugar up to 10.63%. NaOH 50% (b/v) was added to hydrolisat to achieve the fermentation pH. An addition of NaOH increased the concentration of sugar from 9.68-10.63% to11.82-13.25%. Fermentation was carried out to convert sugar to ethanol. Fermentation was done at 35℃, pH 4.5, at 100 rpm for 48 hours. The sugar concentration of hydrolisat which had been added by glucose increased up to 14.91%, after fermented using fermipan 10% (v/v) produced ethanol 1.58%. After distillated at ±80℃ for 1 hour the ethanol concentration increased to 7.01% and after the addition of CaO 10% (b/v) the ethanol concentration became 7.78%. Fermentation with the sugar concentration was 11.82% and S.cerevisiae inoculum's variations 6, 7, 8, 9, 10, and 11% (v/v) produced the ethanol concentration up to 0.128% at 11% (v/v) inoculum's volume. The concentration of ethanol was increased to 0.457% (w/v) after distillated at ±80℃. Fermentation with the comparison of inoculum's types showed that pure S. cerevisiae 11% (v/v) produced ethanol 0.12% and fermipan 11% (v/v) produced ethanol 0.07% with the first sugar concentration was 11.82%. The pure S.cerevisiae 11% (v/v) and fermipan 11% (v/v) which had been added by glucose until its sugar concentration became 17.29% produced ethanol respectively 0.88% and 0.87%. Keywords: Hydrolysis, Fermentation, Waste HVS Paper, H2SO4, S.cerevisiae, Distillation, CaO.

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