Bioetanol merupakan sumber energi alternatif yang kini sedang dikembangkan. Dalam penelitian ini, hasil proses hidrolisis Tandan Kosong Kelapa Sawit (TKKS) digunakan untuk pembuatan bioetanol melalui proses fermentasi. Tujuan dari penelitian ini yaitu mengetahui pengaruh jenis dan volume asam, serta konsentrasi substrat pada proses hidrolisis sehingga didapatkan kondisi hidrolisis yang sesuai untuk proses fermentasi. Proses hidrolisis dilakukan pada konsentrasi substrat 15 %DS, dengan penambahan volume asam sulfat (H2SO4) 98% sebanyak 57 ml pada suhu 105 oC dengan waktu proses selama 120 menit. Hasil analisis konsentrasi gula total menunjukkan sebesar 20% (w/v) dan konsentrasi glukosa sebesar 4,82 % (w/v). Proses fermentasi dilakukan pada pH 5, suhu 30 oC, dan waktu fermentasi 72 jam dengan menggunakan ragi Saccharomyces cerevisiae. Berdasarkan hasil analisis kadar etanol yang dihasilkan adalah 4,37 %. Kata kunci : Hidrolisis, tandan kosong kelapa sawit (TKKS), asam sulfat (H2SO4), bioetanol

Bioethanol is an alternative energy source that is now being developed. In this study, the results of the hydrolysis of Oil Palm Empty Fruit Bunches (OPEFB) was used for the manufacture of bioethanol through fermentation processes. The purpose of this research was to observe the effects of the type and volume of acid, and substrate concentration on the hydrolysis process so the appropriate hydrolysis conditions for the fermentation process could be obtained. Hydrolysis process was carried out at the substrate concentration of 15% DS, with the addition of the volume of 98% sulfuric acid (H2SO4) 57 ml, at temperature of 105 °C with the processing time for 120 minutes. The analysis showed that the concentration of total sugar was 20% (w/v) and concentration of glucose was 4.82% (w/v). The fermentation process was carried out at pH 5, temperature 30 °C, and fermentation time of 72 hours by using the yeast of Saccharomyces cerevisiae. Based on the analysis, the resulting ethanol content was 4.37%. Keyword : Hydrolysis, oil palm empty fruit bunches, sulfuric acid (H2SO4), bioethanol

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